lncRNA-TINCR Functions as a Competitive Endogenous RNA to Regulate the Migration of Mesenchymal Stem Cells by Sponging miR-761

Figure 4

Wnt2 is a potential target of miRNA-761. (a) Using TargetScan for matching with potential target genes, we discovered that the migration-related Wnt2 is one of the targets with miR-761 binding site in its 3UTR region. (b) We synthesized a wild-type 3UTR (WT-Wnt2) with miR-761 binding site and a mutant 3UTR (MU-Wnt2) without. (c) The luciferase reporter activity was remarkably inhibited in the group of WT-Wnt2 with miR-761 mimics, but not in the group of MU-Wnt2 with miR-761 mimics (, ). (d) Real-time PCR results revealed that miR-761 was a negative factor for Wnt2 expression in rMSCs (, ). All data are presented as .

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