Comparison of Chemical Composition and Bioactivities of Essential Oils from Fresh and Dry Rhizomes of Zingiber zerumbet (L.) Smith

Table 5

Cytotoxic properties of FR-EO and DR-EO of Z. zerumbet and zerumbone.

Sample	Incubation time (h)	IC50 (μg/mL)^a
		A549^b	PC-3^c	K562^d	MRC-5^e
FR-EO	24	44.88 ± 1.21	53.32 ± 1.34	35.73 ± 1.72	159.47 ± 9.34
	48	38.64 ± 1.03	21.45 ± 1.18	13.73 ± 0.54	133.82 ± 5.97
	72	14.51 ± 0.61	11.23 ± 0.53	10.48 ± 0.95	106.21 ± 7.34
DR-EO	24	68.06 ± 1.09	77.45 ± 0.46	41.79 ± 1.18	216.99 ± 8.27
	48	56.14 ± 1.76	43.90 ± 1.65	17.22 ± 0.51	164.68 ± 2.71
	72	33.24 ± 0.53	13.83 ± 0.59	14.96 ± 1.18	147.29 ± 4.30
Zerumbone	24	22.40 ± 1.80	30.78 ± 1.31	10.08 ± 0.61	117.96 ± 5.67
	48	19.00 ± 0.72	14.30 ± 1.84	6.24 ± 1.05	79.79 ± 3.17
	72	11.09 ± 0.39	7.66 ± 0.68	4.21 ± 0.84	56.98 ± 1.82
Cisplatin^f	24	5.17 ± 0.44	40.64 ± 1.38	20.36 ± 0.62	14.58 ± 0.91
	48	3.38 ± 0.61	7.64 ± 0.39	11.53 ± 0.91	5.29 ± 0.67
	72	1.91 ± 0.87	2.16 ± 0.67	5.10 ± 0.54	2.99 ± 0.49

^aIC₅₀: the sample concentration reduced cells growth by 50% (after 24-, 48-, and 72-hour incubation), expressed as the mean ± SD of triplicate experiments. ^bHuman lung cancer cell line. ^cHuman prostatic carcinoma cell line. ^dHuman leukemic cell line. ^eHuman fetal lung fibroblasts cell line. ^fCisplatin as positive control.