Phosphatidylinositol-4,5-Biphosphate (PI(4,5)P₂) Is Required for Rapid Endocytosis in Chromaffin Cells

Figure 3

Inhibition of RE by Ab_PI4K in chromaffin cells subjected to one round of stimulation. The following representative capacitance (Cm) traces correspond to (a) untreated cells (control); (b) Heat-inactivated Ab_PI4K (1 mg/ml); (c) Ab_PI4K (1 mg/ml); (d) Ab_PI4K + PI(4,5)P₂. Arrows and bars above traces indicate the timing at which the stimulation protocol was executed to elicit secretion, and the dashed lines are baselines.

(a)

(b)

(c)

(d)