Screening of a Novel Upregulated lncRNA, A2M-AS1, That Promotes Invasion and Migration and Signifies Poor Prognosis in Breast Cancer

Figure 2

A2M-AS1 is upregulated in breast cancer tissues and promotes breast cancer cell proliferation. (a) A2M-AS1 expression in breast cancer tissues and normal tissues was measured by qRT-PCR. (b) A2M-AS1 expression was detected in ER-negative and ER-positive breast cancer tissues. Kaplan–Meier analysis of recurrence-free (c) and metastasis-free (d) survival in two groups divided according to low and high expression levels of A2M-AS1. (e) qRT-PCR revealed endogenous expression of A2M-AS1 and the efficiency of A2M-AS1 knockdown and overexpression in four ER-negative breast cancer cell lines. (f) The effect of A2M-AS1 on cell proliferation was detected by CCK8 assays in ZR-75-30/BT-549 cells with A2M-AS1 knockdown and in MDA-MB-231/Hs578T cells with A2M-AS1 overexpression. GAPDH was used as the internal reference. Data are presented as the mean ± SD of three independent experiments. Notes: KD: A2M-AS1-knockdown group; KD-NC: negative control for the A2M-AS1-knockdown group; OE: A2M-AS1-overexpression group; OE-NC: negative control for the A2M-AS1-overexpression group. , , and .

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