The Role of circRNA-SETD2/miR-519a/PTEN Axis in Fetal Birth Weight through Regulating Trophoblast Proliferation

<div>The effect of circ-SETD2 on trophoblast-like HTR8/SVneo cells. The HTR8/SVneo cells were transfected with circ-SETD2 vector. The mRNA levels and protein levels of circ-SETD2 in HTR8/SVneo cells were measured by qRT-PCR (a) and Western blotting (b), respectively. The proliferation of HTR8/SVneo cells was examined by CCK-8 kit post transfection (c). Transwell assay was performed to analyze the invasive activity of circ-SETD2-overexpressed HTR8/SVneo cells (d). The apoptotic HTR8/SVneo cells were analyzed using Annexin V/PI staining via FACS (e). Relative gene expression was normalized by GAPDH expression. 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BioMed Research International

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Figure 2

Figure 2: The Role of circRNA-SETD2/miR-519a/PTEN Axis in Fetal Birth Weight through Regulating Trophoblast Proliferation 