The Role of circRNA-SETD2/miR-519a/PTEN Axis in Fetal Birth Weight through Regulating Trophoblast Proliferation

Figure 3

The enhanced cellular proliferation and invasion by circ-SETD2 are related to miR-519a/PTEN. miR-519a provides binding sites with circ-SETD2 and PTEN (a). The mRNA levels of miR-519a and PTEN were quantified by qRT-PCR, and Western blotting was used for analysis of protein expression of PTEN (b). The binding between miR-519a and PTEN was confirmed by luciferase assay (c). The mRNA levels and protein levels of PTEN in miR-519a-overexpressed or miR-519a-downregulated HTR8/SVneo cells were measured by qRT-PCR and Western blotting, respectively (d). The proliferation and invasive activity of HTR8/SVneo cells were respectively examined by CCK-8 kit (e) and transwell assay (f) after transfection of cells with miR-519a mimics or inhibitors. Relative genes expression was normalized by GAPDH expression. The data are represented as the (). ; .

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