Follicular Fluid Metabolite Changes in Dairy Cows with Inactive Ovary Identified Using Untargeted Metabolomics
Table 2
Differential follicular fluid metabolites of E and IO cows screened by LC-MS.
ID
Metabolites
RT(s)
VIP value
value
Change trend
Mode
1
Valine
302.69
1.92
0.0001
↓
ESI-
2
L-Glutamine
406.31
1.47
0.0337
↑
ESI+
3
4-Methyl 2-oxo-2-valerate
57.88
1.83
0.0018
↓
ESI-
4
Benzene pyruvic acid
92.35
1.41
0.0330
↓
ESI-
5
2,5-Dihydroxybenzoic acid
61.34
1.44
0.0233
↓
ESI-
6
Choline
280.86
1.70
0.0100
↑
ESI+
7
Lysophosphatidylcholine
211.14
1.85
0.0069
↑
ESI+
8
Phosphatidylcholine
36.10
1.13
0.0403
↑
ESI+
9
Phosphatidylethanolamine
58.74
1.68
0.0153
↑
ESI+
10
Arachidonic acid
46.47
1.45
0.0435
↑
ESI-
11
(5Z, 8Z, 14Z) -11,12-dihydroxy eicosapentaenoic acid -5,8, and 14-trienoic acid
78.28
1.66
0.0098
↓
ESI-
12
Maltose
416.44
1.33
0.0276
↑
ESI-
13
6-Hydroxy-5-methoxyindole glucuronic acid
313.81
1.59
0.0043
↓
ESI+
14
Uric acid
322.52
1.73
0.0202
↑
ESI+
The change trend indicates concentration in the IO group (inactive ovary cows, ) higher or lower than that in the E group (estrus cows, ). RT: retention time; s: second; VIP: variable importance in projection.
