Microarray Data Mining and Preliminary Bioinformatics Analysis of Hepatitis D Virus-Associated Hepatocellular Carcinoma

Figure 5

Validation of the expression data and survival curve of hub genes from the 3 modules using the GEPIA database and functional and pathway enrichment analysis. (a) The box plots that verify whether the expression of these DEGs is consistent with that in the LIHC datasets. Among the downregulated genes, CCL21 and FPR1 (module 1) as well as XAF1 (module 2) are consistent with the HCC datasets, while IFI6, IFI27, and ISG15 (module 2) are not. All 8 upregulated genes (module 3) are consistent with the LIHC datasets. (b) The genes are associated with overall survival whose expression is consistent with that in the LIHC datasets. All 8 upregulated genes are from module 3. (c) The chord diagram showing GO terms and KEGG pathway enrichment with the 8 hub genes involved. (d) The intersection of these 8 genes from PPI network analysis and 320 genes contained in module MEturquoise obtained by WGCNA, 7 potential key genes in the middle part. HCC: hepatocellular carcinoma; GO: gene ontology; KEGG: Kyoto Encyclopedia of Genes and Genomes; BP: biological process; CC: cellular component; MF: molecular function; PPI: protein-to-protein interaction; WGCNA: Weighted Gene Coexpression Network Analysis.

(a)

(b)

(c)

(d)