Vitrification of Dog Skin Tissue as a Source of Mesenchymal Stem Cells

Table 1

Summary of preequilibration and cryoprotectant solution in the present study.

Experimental group	Preequilibration solution	Cryoprotectant solution	Freezing method
Fresh (control)	No	No	No freezing
DPBS	DPBS	DPBS	Two steps
40% EG	20% EG in DPBS	40% EG in DPBS	Two steps
40% Me2SO	20% Me2SO in DPBS	40% Me2SO in DPBS	Two steps
	in DPBS	in DPBS	Two steps
sucrose	sucrose in DPBS	sucrose in DPBS	Two steps

The vitrification process was conducted with two steps: Specifically, samples were incubated for 1 min at 25°C in preequilibration solution and then plunged into liquid nitrogen in cryoprotectant solution.