Research Article
Vitrification of Dog Skin Tissue as a Source of Mesenchymal Stem Cells
Table 2
Lists of primers used for RT-qPCR analysis.
| | Gene name (symbol) | Primer sequence | Product size (bp) | Annealing temperature (°C) |
| | POU class 5 homeobox 1 (OCT4) | F: AACGATCAAGCAGTGACTATTCG | 147 | 60 | | R: AGTAGAGCGTAGTGAAGTGAGG |
| | Sex determining region Y-box 2 (SOX2) | F: AGTCTCCAAGCGACGAAAAA | 189 | 60 | | R: CCACGTTTGCAACTGTCCTA |
| | Nanog homeobox (NANOG) | F: GACCGTCTCTCCTCTTCCTTCC | 157 | 60 | | R: CGTCCTCATCTTCTGTTTCTTGC |
| | BCL2-associated X protein (BAX) | F: TTTGCTTCAGGGTTTCATCC | 146 | 60 | | R: TGTTACTGTCCAGTTCATCTCC |
| | B-cell lymphoma 2 (BCL2) | F: GGGTCATGTGTGTGGAGAGC | 180 | 60 | | R: GCCAGGAGAAGTCAAACAGAGG |
| | Baculoviral IAP repeat containing 5 (BIRC5) | F: ACATTCATCTGGTTGTGCTTTCC | 157 | 60 | | R: CACTTTCTTTGCGGTCTCTTCG |
| | Peroxisome proliferator-activated receptor gamma (PPARγ) | F: GCAAGCACTTCACAAGAAACTACC | 108 | 60 | | R: ATGGGAGTGGTCATCCATTACG |
| | Fatty acid binding protein 4 (FABP4) | F: CTGGGCTCCAGAAGGTCATA | 212 | 60 | | R: GATGATCCTGTTGGGTTTGG |
| | Runt-related transcription factor 2 (Runx2) | F: TCTTCCCAAAGCCAGAGTGG | 167 | 60 | | R: TTTAATAGCGTGCTGCCATTCG |
| | Osteonectin | F: GGTGCTGAGGAAACTGAAGAGG | 180 | 60 | | R: CTTGTTGTCGTTGCTGCATACC |
| | Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) | F: CCCATTCCATCTTCCAGAAA | 196 | 60 | | R: ATCTCACGGTTCCAGTTTGC |
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