Research Article
Multiple Biomarker-Combined Screening for Colorectal Cancer Based on Bisulfate Conversion-Free Detection of Fecal DNA Methylation
Table 2
Primers for PCR amplification.
| | Genes | Primers |
| | SEPT9-F | 5-GTCGGATTTCGCGGTTAACGC-3 | | SEPT9-R | 5-CAACCAACCCAACACCCACCTT-3 | | BMP3-F | 5-TTTGAAAATATTCGGGTTATATACGTCGC-3 | | BMP3-R | 5-ATAAACTCTTCCCCAACAACTACGCGAA-3 | | SFRP2-F | 5-CGGAGCCCCCCGGAGCTGCGC-3 | | SFRP2-R | 5-TGGCAGCCGGCGGCTGGGGCGC-3 | | SDC2-F | 5-AGGAGGAGGGGCGCAGCCGC-3 | | SDC2-R | 5-GCAGAGCGGCGGGAGCGC-3 | | NDRG4-F | 5-GGGTGTCCCCCAGGCTCCGC-3 | | NDRG4-R | 5-GTGGCTTCCGCCTTCTGCGC-3 | | ACTB-F | 5-GATGACCCAGGTGAGTGGCCCGCTACCTC-3 | | ACTB-R | 5-GAGAGAACCAGTGAGAAAGGACGCAG-3 |
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