Multiple Biomarker-Combined Screening for Colorectal Cancer Based on Bisulfate Conversion-Free Detection of Fecal DNA Methylation

Table 4

CT values of markers in reference cell lines detected by bisulfite-free methods.

Genes	Reference DNA concentration	CT (test 1)	CT (test 2)	CT (test 3)
SEPT9	5%	36.46	36.89	37.21
	1%	42.09	41.52	41.33
	0.5%	44.01	—	—
NDRG4	5%	37.92	38.96	37.61
	1%	41.37	41.98	42.18
	0.5%	—	—	44.08
ACTB	5%	31.87	32.14	33.66
	1%	35.53	35.01	36.47
	0.5%	41.99	42.05	42.36