Effect of 1,25-Dihydroxyvitamin D3 on Stem Cells from Human Apical Papilla: Adhesion, Spreading, Proliferation, and Osteogenic Differentiation

Figure 6

SCAPs cell proliferation activity; indicates . The results of CCK-8 detecting the effect of 1,25-dihydroxyvitamin D₃ on the proliferation activity of SCAPs are shown. It seemed that no significant difference in the OD values existed on the first day between the control and experimental groups (). From the 3rd day, the OD value of the osteogenic induction culture+1,25-dihydroxyvitamin D₃ group was considerably higher than that of the normal medium group (). On the 5th day, the cell proliferation activity of the osteogenic induction culture+1,25-dihydroxyvitamin D₃ group was immensely better than that of the ordinary medium group and the osteogenic induction culture group (). On the 7th day, this trend was further expanded, inferring that the 1,25-dihydroxyvitamin D₃ group can greatly promote SCAPs proliferation.