Curcumin Inhibits Replication of Human Parainfluenza Virus Type 3 by Affecting Viral Inclusion Body Formation

Figure 4

Curcumin damages the structural integrity of F-actin. (a, b) HeLa cells were infected with HPIV3_HA-P at an MOI of 1.0, DMSO and curcumin were added at 12 h before sample collection, and cells were collected and fixed 36 h after infection. F-actin was stained with phalloidin-AF488, HA-P was immunostained to visualize IBs, and nuclei were counterstained with DAPI. . The yellow arrow indicates IBs, and quantification for IBs was calculated as described in the legend for Figure 3. Data are from three experiments. Student’s test: . (c, d) HeLa cells were infected with HPIV3_HA-P and treated as described in (a, b). At 36 h after infection, cellular microtubules were stained with rabbit anti-α-tubulin primary antibody and goat anti-rabbit AF488, HA-P was immunostained to visualize IBs, and nuclei were counterstained with DAPI. . The yellow arrow indicates IBs, and quantification for IBs was calculated as described above. Data are from three experiments. Student’s test: .

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