Increased Accuracy to c-Fos-Positive Neuron Counting

Figure 1

The variability of the intensity label of the core label of c-Fos-positive neurons. Photomicrography (200x) of the periaqueductal gray showing the core label of c-Fos-positive neurons of the two animals ((a, b)) submitted (both) to the treatment with MEKR (400 mg/kg; p.o. in the previous study [16]). The numbers below the arrows represent the intensity value of the core label of c-Fos-positive neurons. The values inside the circle represent the average of the intensity value of the background in the selected area limited by the circle.

(a)

(b)