Insulin Receptor Substrate p53 Ameliorates High-Glucose-Induced Activation of NF-κB and Impaired Mobility of HUVECs

Figure 2

DM-induced changes in the expression levels of IRSp53 and gal-3 in the aorta of rats. (a) The expression levels of IRSp53 and gal-3 in the aorta of NC rats or DM rats were investigated by Western blotting using β-actin as a loading control (NC group, ; DM group, ). (b) Representative images of aorta immunofluorescence staining showing levels of IRSp53 and CD31 (20x magnification) (NC group, ; DM group, ); the colocalization ratio of IRSp53 is shown in the bar graph. (c) Representative images of aorta immunofluorescence staining showing levels of gal-3 and CD31 (20x magnification) (NC group, ; DM group, ); the colocalization ratio of IRSp53 is shown in the bar graph. vs. the NC group.

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