MiR-599 Protects Cardiomyocytes against Oxidative Stress-Induced Pyroptosis

Figure 1

H₂O₂ induces pyroptosis in cardiomyocytes. (a) Exposure of neonatal rat cardiomyocytes to different concentrations of H₂O₂ (0, 25, 50, 100 μM) for 24 h. Cell viability was determined by MTT assay. (b) ASC mRNA levels determined by qRT-PCR. (c) ASC protein levels determined by Western blot analysis. Caspase-1 (d) and cleaved-caspase-1 (C-caspase-1) (e) protein levels determined by Western blot analysis. The data are presented as of five independent experiments. , , , vs. Control.

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