LncRNA GSTM3TV2 Promotes Cell Proliferation and Invasion via miR-597/FOSL2 Axis in Hepatocellular Carcinoma

Figure 3

lncRNA GSTM3TV2 is targeted by miR-597. (a) The binding positions of miR-597 on lncRNA GSTM3TV2 were predicted by using miRDB, and the binding site of miR-597 on WT and the mutational lncRNA GSTM3TV2 were displayed. (b) Luciferase reporter system was constructed to measure the interaction between miR-597 and lncRNA GSTM3TV2. (c) The level of lncRNA GSTM3TV2 was measured by Real-time qPCR in HCC cells when transfecting miRNA mimics and ASO. (d) The level of miR-597 was measured by Real-time qPCR tumor and adjacent normal tissues in HCC patients. (e) The relationship between the expression of lncRNA GSTM3TV2 and miR-597 was analyzed by using Pearson’s correlation analysis. (f, g) Cell viability was measured by MTT assay in HCC cell lines. (h, i) Wound healing assay and transwell assay were carried out to detect cell migration and invasion abilities. Data are shown as . All experiments were repeated three times. ; ; . in wound healing assay. in a transwell assay.

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