Proteome Analysis of the Hypothalamic Arcuate Nucleus in Chronic High-Fat Diet-Induced Obesity

Figure 3

Volcano plot of DEPs. (a) The statistical significance (−log₁₀ value between the mean iBAQ intensities of the LFD and HFD groups) is plotted against the difference in expression intensity (log₂ fold change between the mean iBAQ intensities of the LFD and HFD groups) in a volcano plot. Green dots represent Frs2 and Shc3 in the neurotrophin signaling pathway. Data were filtered in accordance with the cut-off value of and a or ≤1.0/1.5. Bar graphs indicate the mean and SEM of the iBAQ intensity (logarithmic scale) in each group of proteins. (b) Frs2 (, ), (c) Shc3 (, ), and (d) Mapk14 (, ). LFD: low-fat control diet; HFD: high-fat diet; iBAQ: intensity-based absolute qualification; SEM: standard error of the mean; Shc3: SHC adaptor protein 3; Frs2: fibroblast growth factor receptor substrate 2; Mapk14: mitogen-activated protein kinase 14. .

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