Genetic Association of PPARGC1A Gene Single Nucleotide Polymorphism with Milk Production Traits in Italian Mediterranean Buffalo

Table 1

Primer sequences used for amplification of genomic sequence and identified SNPs for the PPARGC1A gene in Italian Mediterranean buffalo.

Primer set	Primer sequence	Length (bases)	PCR product size (bp)	Identified SNP	SNP location
PPARGC1AF1; PPARGC1AR1	5-TGCTGTGTTGGGGGAAGATG-3; 3-GGGCTGAAAGAGATGAGAGAGTGA-5	20; 24	663	g.-78A>G	Promotor
PPARGC1AF2; PPARGC1AR2	5-AGATCCCACATGCCACAACT-3; 3-GACAGCAAAGCAAGAACCCA-5	20; 20	780	g.224651G>C	Intron 1
PPARGC1AF3; PPARGC1AR3	5-TTTCTCGCTTTCCCTCCTTC-3; 3-ACGCTCCGTTTTCACTCACA-5	20; 20	654	g.286986G>A	Intron 4
PPARGC1AF4; PPARGC1AR4	5-AGTGGACACGAGGAAAGGAAG-3; 3-GGGTGGGTTTTGACAAGGTT-5	21; 20	724	g.304050G>A	Exon 8
PPARGC1AF5; PPARGC1AR5	5-TGAACACATGCACCCCATCAT-3; 3-CGTGCCAGGAGTTTGGTTGT-5	21; 20	789	g.325647G>A; g.325817T>C; g.325997G>A	Exon 13; Exon 13; Exon 13