AAV Induced Expression of Human Rod and Cone Opsin in Bipolar Cells of a Mouse Model of Retinal Degeneration

Figure 3

Rhodopsin expression in example rd1 eyes following injection with AAV2/8 (BP2) 4xGrm6-SV40.RHO-http://YFP.WPRE.pA. Example tissue whole mounts revealed RHO-YFP expression in rd1 eyes after intravitreal injection (a) and subretinal injection (b) with retinal sections showing expression of the RHO-YFP fusion protein in retinal bipolar cells in the distal part of the inner nuclear layer (c). White arrows indicate elected cells with bipolar cell morphology, including long axon extensions towards the IPL with strong expression also evident in the IPL. GCL: ganglion cell layer; IPL: inner plexiform layer; INL: inner nuclear layer.

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