AAV Induced Expression of Human Rod and Cone Opsin in Bipolar Cells of a Mouse Model of Retinal Degeneration

Figure 4

Medium-wave cone opsin expression in example rd1 eyes following injection with AAV2/8 (BP2) 4xGrm6-SV40.MWC-http://YFP.WPRE.pA. Example tissue whole mounts revealed MWC-YFP fusion protein expression (a) with example retinal sections showing expression of the MWC-YFP fusion protein in retinal bipolar cells in the distal part of the inner nuclear layer (b, c). White arrows indicate elected cells with bipolar cell morphology, including axon extensions towards the IPL. Occasional suspected amacrine or horizontal cell expression was also observed (d), orange arrow. GCL: ganglion cell layer; IPL: inner plexiform layer; INL: inner nuclear layer.

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