A Potential miRNA-mRNA Network for Dementia and Hernia Crosstalk

Figure 6

miR-4451 downregulates the expression of PI16. The relative expression of miR-4451 and PI16 in normal and hernia, respectively, was detected by qPCR (a, b). Pattern plots of PI16 wild type and miR-4451 binding with PI16 mutant groups (c). miR-4451 was determined as a target miRNA for PI16 using RNAhybrid 2.12. The minimum free energy was 24 kcal/mol (d). Expression levels of mir-32-3p in HEK-293 cells were detected by RT-qPCR after transfection of cells with mir-32-3p mimics (e). Pattern diagram of miR-4451 possibly acting as a sponge for PI16 and downregulating PI16 expression (f).

(a)

(b)

(c)

(d)

(e)

(f)