Oridonin Promotes Apoptosis and Restrains the Viability and Migration of Bladder Cancer by Impeding TRPM7 Expression via the ERK and AKT Signaling Pathways

Figure 3

Oridonin inactivates the AKT and ERK signaling pathways by inhibiting TRPM7 expression. (a–d) Western blotting was performed to determine the expression of TRPM7, p-AKT, and p-ERK after the T24 cells were treated with 0, 2, and 3 μM oridonin. (b–e) RT-PCR was performed to determine the mRNA expression of FOXO3 after T24 cells were treated with 0, 2, and 3 μM oridonin. (f) T24 cells were infected with TRPM7-overexpressing plasmids, and then, TRPM7, p-AKT, and p-ERK expression was examined after treatment with and without 3 μM oridonin. , , , and vs. the untreated control (0 μM).

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