VX765, a Specific Caspase-1 Inhibitor, Alleviates Lung Ischemia Reperfusion Injury by Suppressing Endothelial Pyroptosis and Barrier Dysfunction

Figure 3

I/R induced endothelial pyroptosis in vivo. (a–h) Mouse lung tissue and serum were collected from the control and I/R groups for the following experiments. (a–c) Caspase-1, GSDMD, and IL-1β mRNA levels measured by RT-qPCR. (d) Protein levels of pro-GSDMD, GSDMD-N, pro-caspase-1, and caspase-1 P20 by detected by Western blotting. (e, f) IL-1β protein level in lung tissue and serum measured by ELISA. (g, h) Costaining of CD31 (green) with caspase-1 or GSDMD (red) and the vascular endothelium (yellow arrow). DAPI was used to stain the cell nuclei. Scale bar, 200 μm. The data are expressed as ( per group). vs. control.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)