VX765, a Specific Caspase-1 Inhibitor, Alleviates Lung Ischemia Reperfusion Injury by Suppressing Endothelial Pyroptosis and Barrier Dysfunction

Figure 4

OGD/R-induced endothelial pyroptosis in vitro. (a–h) PMVECs and supernatant were collected from the control and OGD/R groups for the following experiments. (a–c) Caspase-1, GSDMD, and IL-1β mRNA levels measured by RT-qPCR. (d) Protein levels of pro-GSDMD, GSDMD-N, pro-caspase-1, and caspase-1 P20 detected by Western blotting. (e) Protein level of IL-1β in the cell supernatant measured by ELISA. (f) LDH release rate. (g, h) Immunofluorescence staining for caspase-1 or GSDMD (red). DAPI was used to stain the cell nuclei. Scale bar, 200 μm. The data are expressed as . Each experiment was performed independently at least 3 times. vs. control.

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