VX765, a Specific Caspase-1 Inhibitor, Alleviates Lung Ischemia Reperfusion Injury by Suppressing Endothelial Pyroptosis and Barrier Dysfunction

<div>VX765 treatment attenuated I/R-induced endothelial pyroptosis. (a–h) Mouse lung tissue and serum were collected from each group for the following experiments. (a–c) Caspase-1, GSDMD, and IL-1<i>β</i> mRNA levels measured by RT-qPCR. (d) Protein levels of pro-GSDMD, GSDMD-N, pro-caspase-1, and caspase-1 P20 detected by Western blotting. (e, f) IL-1<i>β</i> protein level in lung tissue and serum measured by ELISA. (g, h) Colabeled CD31 (green) with caspase-1 or GSDMD (red). DAPI was used to stain the cell nuclei by immunofluorescence; vascular endothelium (yellow arrow), scale bar, 200 <i>μ</i>m. 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BioMed Research International

fig7

Figure 7

Figure 7: VX765, a Specific Caspase-1 Inhibitor, Alleviates Lung Ischemia Reperfusion Injury by Suppressing Endothelial Pyroptosis and Barrier Dysfunction 

Figure 7 | VX765, a Specific Caspase-1 Inhibitor, Alleviates Lung Ischemia Reperfusion Injury by Suppressing Endothelial Pyroptosis and Barrier Dysfunction 