VX765, a Specific Caspase-1 Inhibitor, Alleviates Lung Ischemia Reperfusion Injury by Suppressing Endothelial Pyroptosis and Barrier Dysfunction

<div>Effects of VX765 on PMVEC pyroptosis. (a–h) PMVECs and supernatant were collected from the controls and OGD/R group for the following experiments. (a–c) Caspase-1, GSDMD, and IL-1<i>β</i> mRNA levels. (d) Protein levels of pro-GSDMD, GSDMD-N, pro-caspase-1, and caspase-1 P20 measured by WB. (e) The protein level of IL-1<i>β</i> in the cell supernatant. (f) LDH release rate. (g, h) Immunofluorescence staining for caspase-1 or GSDMD (red). DAPI was used to stain the cell nuclei. Scale bar, 200 <i>μ</i>m. 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BioMed Research International

fig8

Figure 8

Figure 8: VX765, a Specific Caspase-1 Inhibitor, Alleviates Lung Ischemia Reperfusion Injury by Suppressing Endothelial Pyroptosis and Barrier Dysfunction 

Figure 8 | VX765, a Specific Caspase-1 Inhibitor, Alleviates Lung Ischemia Reperfusion Injury by Suppressing Endothelial Pyroptosis and Barrier Dysfunction 