Topical Delivery of Geranium/Calendula Essential Oil-Entrapped Ethanolic Lipid Vesicular Cream to Combat Skin Aging

Table 5

Skin irritation study scores for primary irritation and primary irritation index measured at 24, 48, and 72 hrs after applying the cream formulations on the rat skin.

(a)

Skin reaction	Time (hrs)	Group 1 (cream base)			Group 2 (formulation GC3)			Group 3 (formulation CC3)
		Rat-1	Rat-2	Rat-3	Rat-1	Rat-2	Rat-3	Rat-1	Rat-2	Rat-3
Erythema	24	1	1	1	1	1	1	1	0	0
	48	0	0	0	0	0	0	0	0	0
	72	0	0	0	0	0	0	0	0	0
Edema	24	0	0	0	1	0	1	1	1	0
	48	0	0	0	0	0	0	0	0	0
	72	0	0	0	0	0	0	0	0	0
Score of primary irritation		0.16	0.16	0.16	0.33	0.16	0.33	0.33	0.16	0.0
Primary irritation index		0.16			0.27			0.16

(b)

Skin reaction	Time (hrs)	Group 4 (GEO6)			Group 5 (CEO6)
		Rat-1	Rat-2	Rat-3	Rat-1	Rat-2	Rat-3
Erythema	24	1	1	1	1	1	1
	48	1	0	1	1	1	1
	72	0	0	0	0	0	0
Edema	24	1	1	1	1	1	0
	48	1	0	1	0	1	0
	72	0	0	0	0	0	0
Score of primary irritation		0.66	0.33	0.66	0.50	0.66	0.33
Primary irritation index		0.55			0.49