Comprehensive Profile Analysis of Differentially Expressed circRNAs in Glucose Deprivation-Induced Human Nucleus Pulposus Cell Degeneration

<div>Expression and characterization of circ_0075062. (a, b) The expression level of circ_0075062 in degenerated NP tissues and NP cells cultured under glucose deprivation was detected by qRT-PCR. (c) Sanger sequencing of circ_0075062 showed the back-splice junction. (d) circ_0075062 is derived from exon 4 of the STC2 gene. Divergent primer detected circ_0075062 in cDNA but not gDNA. (e) The relative expressions of circ_0075062 and linear 0075062 in NP cells were determined by qRT-PCR after transfecting with the circ_0075062 interference plasmid. (f, g) After transfecting with the circ_0075062 interference plasmid, the protein expression level of collagen II, aggrecan, and MMP-13 was analyzed with western blotting under glucose deprivation for 24 h. 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BioMed Research International

fig5

Figure 5

Figure 5: Comprehensive Profile Analysis of Differentially Expressed circRNAs in Glucose Deprivation-Induced Human Nucleus Pulposus Cell Degeneration 

Figure 5 | Comprehensive Profile Analysis of Differentially Expressed circRNAs in Glucose Deprivation-Induced Human Nucleus Pulposus Cell Degeneration 