Research Article
Production and Partial Characterization of α-Amylase Enzyme from Marine Actinomycetes
Table 9
Progression of purification fold for amylase production using Streptomyces rochei HMM 13.
| | (U/ml) | (mg/ml) | SA (U/mg) | Ap (mm) | Protein recovery | P. fold | Yield% |
| Basal medium | 0.227 | 0.407 | 56 | 24 | | 1 | 100 | Plackett-Burman | 30.001 | 10.82 | 277 | 33 | 2658 | 3.97 | 64.47 | Box-Behnken | 64.895 | 8.803 | 737 | 45 | 2162 | 12.22 | 24.23 | Ammonium sulfate (90%) | 67.91 | 7.22 | 940 | — | 1773 | 15.58 | 18.9 | Sephadex G-50 | | | | | | | | First peak | 22.442 | 2.305 | 973 | | 566.34 | 16.12 | 19.56 | Second peak | 14.364 | 1.261 | 1138 | | 309.8 | 18.85 | 22.87 | Third peak | 14.966 | 1.68 | 890 | | 412.78 | 14.74 | 17.88 |
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Note: (U/ml): activity; (mg/ml): protein; SA (U/mg): specific activity; Ap (mm): agar diffusion plate; P. fold: purification fold. |