A Hypercoagulable Hematological Metastasis Breast Cancer Model

Table 2

Physical characteristics (concentration and size) of supernatants. (1) Within a certain period of culture time (30 min-24 h), extension of the culture time promoted release. (2) Coculture promoted release. (3) There were no significant differences in EV size among the supernatants.

Sample	Size(nm)	Concentration
4T1 (30 min)	61.14 ± 16.45	5.45 × 10^9
4T1 (24 h)	57.63 ± 16.91	9.47 × 10^9
PLT (30 min)	64.26 ± 27.31	2.39 × 10^10
PLT (24 h)	57.35 ± 19.73	3.28 × 10^10
4T1+PLT (30 min)	61.79 ± 24.34	2.97 × 10^10
4T1 + PLT (24 h)	57.37 ± 19.55	5.73 × 10^10

4T1: murine breast cancer cell line. PLT: platelets obtained from a BALB/c mouse. 4T1 + PLT: coculture of the murine breast cancer cell line and the PLTs obtained from the BALB/c mouse.