The Production of Standardized Samples with Known Concentrations for Severe Acute Respiratory Syndrome Coronavirus 2 RT-qPCR Testing Validation for Developing Countries in the Period of the Pandemic Era

Table 3

Intermediate precision of Ct value per dilution concentration of synthesized RNA.

No.	Repeatability	Numbers (copy/μL)	Mean (Ct value)	Log copy Ct value 1	Log copy Ct value 2	Log copy Ct value 3	Average log copy	Average SD log copy	RSD % (log copy)
1	Day 1	48,300	23.58	4.47	4.24	4.70	4.47	0.23	5.11
2	Day 2	48,300	22.50	4.76	4.63	4.89	4.76	0.13	2.80
3	Day 3	48,300	22.87	4.66	4.39	4.93	4.66	0.27	5.83
4	Day 1	4,830	27.15	3.36	3.31	3.82	3.50	0.28	8.11
5	Day 2	4,830	25.67	4.44	3.85	3.41	3.90	0.52	13.30
6	Day 3	4,830	26.03	3.53	3.80	4.07	3.80	0.27	7.15
7	Day 1	483	30.70	2.26	2.80	2.53	2.53	0.27	10.73
8	Day 2	483	29.28	3.19	2.92	2.65	2.92	0.27	9.31
9	Day 3	483	29.89	2.75	3.02	2.48	2.75	0.27	9.87
10	Day 1	48.3	34.79	1.42	1.56	1.29	1.42	0.14	9.56
11	Day 2	48.3	33.18	1.67	1.89	2.02	1.86	0.18	9.59
12	Day 3	48.3	34.10	1.52	1.61	1.69	1.61	0.08	5.24