The Production of Standardized Samples with Known Concentrations for Severe Acute Respiratory Syndrome Coronavirus 2 RT-qPCR Testing Validation for Developing Countries in the Period of the Pandemic Era

Table 6

Concentration of RNA extracted from the SARS-CoV-2 virus.

No.	Ct values			Log copy; Day 1	Log copy; Day 2	Log copy; Day 3	Average log copy	SD log copy	RSD % log copy	Copy/reaction (5 μL)
	Day 1	Day 2	Day 3
1	19.94	20.27	20.10	5.46	5.37	5.41	5.41	0.04	0.83	259,226
2	20.26	20.03	20.08	5.37	5.43	5.42	5.41	0.03	0.61	255,577
3	19.69	20.19	20.55	5.52	5.39	5.29	5.40	0.12	2.17	258,182
4	23.80	23.53	24.06	4.41	4.48	4.34	4.41	0.07	1.63	25,852
5	23.61	23.58	23.78	4.46	4.47	4.41	4.45	0.03	0.66	28,003
6	23.59	23.60	23.80	4.46	4.46	4.41	4.44	0.03	0.72	27,895
7	26.78	26.97	27.19	3.60	3.55	3.49	3.54	0.06	1.57	3,514
8	26.96	27.09	27.07	3.55	3.51	3.52	3.53	0.02	0.54	3,369
9	27.13	26.91	27.29	3.50	3.56	3.46	3.51	0.05	1.48	3,238
10	27.87	28.65	28.24	3.30	3.09	3.20	3.20	0.11	3.32	1,607
11	28.26	28.39	28.55	3.20	3.16	3.12	3.16	0.04	1.25	1,441
12	27.89	28.99	28.45	3.30	3.00	3.14	3.15	0.15	4.75	1,455
13	29.17	30.65	27.46	2.95	2.55	3.41	2.97	0.43	14.61	1,276
14	29.17	30.13	29.26	2.95	2.69	2.92	2.85	0.14	5.05	738
15	29.17	30.09	29.09	2.95	2.70	2.97	2.87	0.15	5.26	774
16	30.41	31.77	31.55	2.61	2.24	2.30	2.38	0.20	8.32	261
17	30.21	32.30	31.43	2.67	2.10	2.33	2.37	0.29	12.06	268
18	30.27	31.49	30.96	2.65	2.32	2.46	2.48	0.17	6.71	315
19	34.23	33.16	34.09	1.57	1.86	1.61	1.68	0.16	9.39	50
20	34.51	33.66	34.04	1.50	1.73	1.63	1.62	0.12	7.16	42
21	33.81	33.70	34.56	1.69	1.72	1.48	1.63	0.13	7.80	44
22	36.42	35.78	36.23	0.98	1.15	1.03	1.05	0.09	8.48	11
23	37.03	37.19	37.34	0.81	0.77	0.73	0.77	0.04	5.47	6
24	38.03	38.19	38.34	0.54	0.50	0.46	0.50	0.04	8.46	3