Research Article

The Production of Standardized Samples with Known Concentrations for Severe Acute Respiratory Syndrome Coronavirus 2 RT-qPCR Testing Validation for Developing Countries in the Period of the Pandemic Era

Table 7

Summary of the criteria for synthesized RNA.

CriteriaResultRequired

LOD~3 copies/reaction
Linearity0.980.98
Accuracy1.01-2.51%<25%
Precision
 Repeatability (intraprecision)0.77-8.33%
 Interprecision2.80-13.30%