Apoptosis Induced by Ziziphora tenuior Essential Oil in Human Colorectal Cancer Cells

Figure 4

Ziziphora essential oil- (ZEO-) induced apoptosis includes an increase in caspase 3 and 9 and a decrease in Bcl-2 and Bax expressions in HT-29 cells. (a) Light microscopic pictures (×100 magnification) with arrows represent the morphological changes caused by apoptosis in the examined HT-29 cells. (b) Bax, Bcl-2, C3, and C9 expression results obtained by western blot in HT-29 cells. (c) mRNA, Bax, Bcl-2, C3, and C9 results obtained by RT-PCR.

(a)

(b)

(c)