Comparison of Pluripotency, Differentiation, and Mitochondrial Metabolism Capacity in Three-Dimensional Spheroid Formation of Dental Pulp-Derived Mesenchymal Stem Cells

Figure 5

The oxygen consumption rate of 2D cultured and 3D cultured DPSCs. (a) The 48-hour and 72-hour 3D cultured DPSCs (3D 48 h and 3D 72 h, respectively) revealed decreased mitochondrial respiration compared to 2D and 24-hour 3D cultured DPSCs (2D and 3D 24 h, respectively). (b) The quantification of basal respiration, spare respiratory capacity, and ATP production was decreased in 3D 48 h and 3D 72 h as compared with 2D and 3D 24 h. The values of data in OCR represented ( wells in independent experiments). Asterisk () indicates statistical differences between groups ().

(a)

(b)