BioMed Research International

Research Article

Comparison of Pluripotency, Differentiation, and Mitochondrial Metabolism Capacity in Three-Dimensional Spheroid Formation of Dental Pulp-Derived Mesenchymal Stem Cells

Table 2

Lists of primers used in RT-qPCR.
Gene namePrimer sequenceProduct size (bp)Anneal. temp (°C)Reference
OCT4F: ACTATCATTGATGCCCCAGGAC
R: ACACCAGCAGCAACAATCAG		12860NM_021130.3
SOX2F: CACCCACAGCAAATGACAGC
R: GTCCCCCAAAAAGAAGTCCAG		12060NM_021130.3
NANOGF: TGCAACCTGAAGACGTGTG
R: TGCAACCTGAAGACGTGTG		15360NM_024865.2
FABP4F: TGAGATTTCCTTCATACTGG
R: TGGTTGATTTTCCATCCCAT		12860NM_001442.2
LPLF: AGACACAGCTGAGGACACTT
R: GCACCCAACTCTCATACATT		13760NM_001442.2
PPARγF: TTGCTGTCATTATTCTCAGT
R: GAGGACTCAGGGTGGTTCAG		12460AB565476.1
Runx2F: CCTTGGGAAAAATTCAAGCA
R: AACACATGACCCAGTGCAAA		18160NM_001015051
OPN (osteopontin)F: CATCACCTCACACATGGAAAGC
R: CTGACTCGTTTCATAACTGTCC		11560NM_001251830.1
ON (osteonectin)F: GTGCAGAGGAAACCGAAGAG
R: AAGTGGCAGGAAGAGTCGAA		17060J03040.1
YWHAZF: ACGAAGCTGAAGCAGGAGAAG
R: TTTGTGGGACAGCATGGATG		11160BC108281.1