BEZ235 Increases the Sensitivity of Hepatocellular Carcinoma to Sorafenib by Inhibiting PI3K/AKT/mTOR and Inducing Autophagy

<div>The effect of various treatments on the cell cycle in Huh7 or Huh7<sup>R</sup>, as determined with flow cytometry and western blot. (a) Huh7 or Huh7<sup>R</sup> cells stained with propidium iodide after treatment for 24 h. (SFB, 4 <i>μ</i>M; BEZ235, 0.25 <i>μ</i>M; SFB, 4 <i>μ</i>M + BEZ, 0.25 <i>μ</i>M). Cell-cycle distribution was analyzed by flow cytometry. (b) Western blot analysis revealed the expression of the key G1-phase regulatory protein after treatment for 24 h: cyclin-D1 and p-Rb and p-GSK3<i>β</i>. (SFB, 4 <i>μ</i>M; BEZ235, 0.25 <i>μ</i>M; and SFB, 4 <i>μ</i>M + BEZ, + 0.25 <i>μ</i>M). 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SFB: sorafenib; BEZ: BEZ235; SFB + BEZ: sorafenib combined with BEZ235.</div>

BioMed Research International

fig3

Figure 3

Figure 3: BEZ235 Increases the Sensitivity of Hepatocellular Carcinoma to Sorafenib by Inhibiting PI3K/AKT/mTOR and Inducing Autophagy 