miR-143-3p Inhibits the Differentiation of Osteoclast Induced by Synovial Fibroblast and Monocyte Coculture in Adjuvant-Induced Arthritic Rats

Figure 1

Osteoclast differentiation, morphological characteristics, and miRNA-143-3p expression in RA and osteoclast-like cells. (a) miR-143-3p expression in RA patients and healthy controls determined by qPCR analysis (data were expressed as the of five independent experiments). (b) miR-143-3p expression in normal monocytes and osteoclasts detected by qPCR analysis (data were expressed as the of three independent experiments). The monocytes (c) were cocultured with synovial fibroblasts (d) of AIA rats, and the monocytes began to differentiate after 5-7 days of coculturing (multinucleated cells appeared with flaky or filamentous pseudopodia) (e) (200x magnification). They differentiated into osteoclast-like cells after 12-14 days of coculturing (f) (200x magnification). (g) Osteoclast-like cells were identified as osteoclasts by TRAP staining (200x magnification). (h) A scanning electron microscope showed that osteoclast-like cells had bone resorption function (4000x magnification). compared with the control group.

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