miR-143-3p Inhibits the Differentiation of Osteoclast Induced by Synovial Fibroblast and Monocyte Coculture in Adjuvant-Induced Arthritic Rats

Figure 3

miR-143-3p expression was negatively correlated with differentiation-related cytokines secretion. Levels of M-CSF and RANKL in culture supernatant of cocultured cells which transfected with miR-143-3p mimics or inhibitors in the early (coculture for 7 days) or later stage (coculture for 14 days) of osteoclast differentiation were detected by ELISA. M-CSF levels in culture supernatant of cocultured cells in the early (a) or later (b) stage of osteoclast differentiation. RANKL levels in culture supernatant of cocultured cells in the early (c) or later (d) stage of osteoclast differentiation. Data were expressed as the of three independent experiments. and compared with the negative control group.

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