miR-143-3p Inhibits the Differentiation of Osteoclast Induced by Synovial Fibroblast and Monocyte Coculture in Adjuvant-Induced Arthritic Rats

Figure 4

miR-143-3p regulated the activation of MAPK proteins in the process of osteoclast differentiation. The expression of JNK, ERK1/2, p38MAPK, p-JNK, p-ERK1/2, and p-p38MAPK in osteoclasts was detected by western blot after miR-143-3p mimics were transfected in the early (coculture for 7 days) (a) or later (coculture for 14 days) (b) stage of osteoclast differentiation. The expression of JNK, ERK1/2, p38MAPK, p-JNK, p-ERK1/2, and p-p38MAPK in osteoclasts was detected after miR-143-3p inhibitors were transfected in the early (c) or later (d) stage of osteoclast differentiation. Data were expressed as the of three independent experiments. and compared with the negative control group.

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