miR-143-3p Inhibits the Differentiation of Osteoclast Induced by Synovial Fibroblast and Monocyte Coculture in Adjuvant-Induced Arthritic Rats

Figure 5

MAPK inhibitors reduced the level of cytokines in culture supernatants of coculture cells and upregulated the expression of miR-143-3p in osteoclasts. Levels of M-CSF and RANKL in culture supernatants of coculture cells in the early (coculture for 7 days) (a, b) or later (coculture for 14 days) (c, d) stage of osteoclast differentiation with MAPK inhibitors detected by ELISA. miR-143-3p expression of osteoclasts in the early (e) or later (f) stage of differentiation with MAPK inhibitors by qPCR analysis. Data were expressed as the of three independent experiments. and compared with the control group.

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