Optimization of Preanalytical Variables for cfDNA Processing and Detection of ctDNA in Archival Plasma Samples

Figure 3

Overview of cfDNA yield at each time point for six volunteers. (a) EDTA BCTs at centrifuge regime (first bars without pattern), Streck BCTs at centrifuge regime (second bars with pattern), EDTA BCTs at (third bars with pattern). Bars represent the mean value of technical duplicates for each time point with a standard deviation. The yield of total cfDNA from each of the volunteers and at each time point is shown in the order of one to six, showing results from both Streck BCTs and EDTA BCTs centrifuged at , and . (b) The panel shows mean with standard deviation of cfDNA yield for all time points where significant difference in cfDNA yield is between EDTA and Streck BCTs at 24 h time point before plasma separation, freezing, and cfDNA purification (Wilcoxon signed-rank test).

(a)

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