Factors Associated with Promoted Proliferation of Osteosarcoma by Peptidylarginine Deiminase 4

Figure 2

The effect of PADI4 on the markers of Wnt/β-catenin and MEK/ERK signaling. (a) Expressions of Wnt/β-catenin and MEK/ERK signaling markers were detected by Western blot after treatment with U2OS cells with Cl-amidine for the indicated time. (b, c) Protein levels of markers involved in Wnt/β-catenin and MEK/ERK signaling were detected by Western blot after PADI4 knockdown or overexpression in both U2OS (b) and Saos-2 (c) cells. (d, e) Quantitative analysis of protein expression levels for markers demonstrated in both U2OS (d) and Saos-2 (e) cells. (f, g) mRNA expression of markers involved in Wnt/β-catenin signaling was analyzed by RT-PCR after Cl-amidine treatment in both U2OS (f) and Saos-2 (g) cells. (h, i) Cell viability detection was performed by CCK8 assay after transfecting PADI4 with or without DKK1 plasmids for the indicated time in both U2OS cells (h) and Saos-2 cells (i).

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