Research Article
Interferon-Induced Transmembrane Protein 3 Expression Upregulation Is Involved in Progression of Hepatocellular Carcinoma
Table 1
Antibodies and sequences used for western blotting, qRT-PCR, and shRNA for LV-IFITM3 and control LV-NC.
| | Protein | Mfrs./made in | Cat no. |
| | Primary antibodies | | | | p-PI3K | Cell Signaling Technology/USA | 4228P | | P-AKT | Cell Signaling Technology/USA | 4060P | | p-mTOR | Cell Signaling Technology/USA | 5536P | | PI3K | Cell Signaling Technology/USA | 4255 | | AKT | Cell Signaling Technology/USA | 9272 | | mTOR | Cell Signaling Technology/USA | 2972 | | GAPDH | Sigma/USA | G9545 | | Vimentin | Cell Signaling Technology/USA | 5741S | | IFITM3 | Cell Signaling Technology/USA | 59212S | | Second antibodies | | | | HRP-conjugated anti-mouse IgG | Zhongshan Boil Tech Co./China | IH-0031 | | HRP-conjugated anti-rabbit IgG | Zhongshan Boil Tech Co./China | IH-0011 | | Gene | | Primer sequences | | | RT-qPCR | | | | | IFITM3 | Forward | 5-AGATGGTTGGCGACGTGAC-3 | | | Reverse | 5-AGGCCTGGAAGATCAGCACT-3 | | | GAPDH | Forward | 5-TGAAGGTCGGAGTCAACGGA-3 | | | Reverse | 5-CCTGGAAGATGGTGATGGGAT-3 | | | AKT | Forward | 5-TGTGGACCAACGTGAGGCTC-3 | | | Reverse | 5-AGGCAGCGGATGATGAAGGT-3 | | | PI3K | Forward | 5-GGAAGCAGCAACCGAAACAA-3 | | | Reverse | 5-AGAGCAGGCATAGCAGCCCT-3 | | | mTOR | Forward | 5-GCATCAGGACCTCTTCTCCT-3 | | | Reverse | 5-GCCCGACTGTAACTCTCTCC-3 | | | shRNA | | | | | LV-IFITM3 | | 5-GTGCTGATCTTCCAGGCCTA-3 | | | LV-NC | | 5-CCTCGTTCACCGCCGTCGCG-3 | |
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