The Mechanism Exploration of Follicular Fluids on Granulose Cell Apoptosis in Endometriosis-Associated Infertility

Figure 5

The apoptosis rates of TI-GCs analyzed by flow cytometry. Group A: TI-GCs incubated in basal medium (DMEM/F-12 supplemented with 10% fetal bovine serum); Group B: TI-GCs incubated in basal medium with 9% TI-FFs; and Group C: TI-GCs incubated in basal medium with 9% EI-FFs. After being incubated 48 h, all of the three groups were tested by flow cytometry and the apoptosis rates were compared between groups. As shown in the chart, the apoptosis rate of Group C was obviously higher than those of Group A and Group B. These suggest that 9% EI-FFs have a significant effect on TI-GC apoptosis.