Research Article

[Retracted] CD4-Targeted T Cells Rapidly Induce Remissions in Mice with T Cell Lymphoma

Figure 1

Generation of CD4-CAR-T cells. (a) Diagram of recombinant plasmid pCD4-CAR containing a single-chain antibody fragment (scFv) specific for CD4 antigen, costimulatory factor CD28 fragment, and intracellular signal transduction domain of CD3 fragments. H indicates hinge region; TM indicates transmembrane region. (b) Identification of correct transgene insert by enzyme digestion and electrophoresis. Plasmid was digested with EcoRI and BamHI. 1.5 kb band indicates insert fragment and 8.6 kb band indicates vector backbone. (c) Flow cytometry analysis for the CD4-CAR modified T cells after Lv.CD4-CAR vector transduction. The positive engineered T cells were analyzed by measuring protein L binding activity to kappa light chain of scFv on CD4-CAR-T cells. Control indicated that T cells were infected with null reporter vector expressing luciferase.
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