Tetrahydrocurcumin Ameliorates Skin Inflammation by Modulating Autophagy in High-Fat Diet-Induced Obese Mice

Figure 4

Beclin 1, Atg5, and LC3 expressions in RD, HFD, and HFD+T100 mouse skin. (a–c) qRT-PCR analysis of (a) BECN1, (b) Atg5, and (c) LC3 expressions in the skin of RD (), HFD (), and HFD+T100 () mice. Relative mRNA expression, determined by qRT-PCR, was normalized to GAPDH expression. Fold differences in the expression were calculated using the comparative CT method and standardizing against the RD value. (d–g) Western blotting analysis of the skin from RD (), HFD (), and HFD+T100 () mice for Beclin 1, Atg5, and LC3 protein expressions. Data were normalized to GAPDH expression. The results are representative of three independent experiments. (e–g) Differences in western blot quantification were determined. Data are presented as of three independent experiments. All comparisons utilized one-way ANOVA and Tukey’s post hoc tests. , , and . RD: regular diet; HFD: high-fat diet; HFD+T100: high-fat diet+tetrahydrocurcumin (100 mg/kg).

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