In Vivo Antitumor Effect against Murine Cells of CT26 Colon Cancer and EL4 Lymphoma by Autologous Whole Tumor Dead Cells

Figure 1

ICBs-EL4 characterization. (a) Size and morphology of live EL4 cells and ICBs-EL4 evaluated by confocal microscopy using CellTraker™ CMTPX Red and Hoechst 33342 shown at the top and bottom panels, respectively. (b) EL4 live cells and ICBs-EL4 area were measured, calculated, and represented as . (c) The ability of DCs to phagocyte ICBs-EL4 was evaluated using syngeneic BM-DCs cells (DCs) generated as described in methods. DCs were challenged with ICBs-EL4 stained with CellTraker™ CMTPX Red. Representative histograms show the CD11c+ population that acquired CMTPX fluorescence from the ICBs-EL4 (red peak), negative control is showed with DCs alone (black peak) and DCs-ICBs-EL4 without staining (grey peak). Latex beads were used as positive control (peak blue). 7-AAD+ cells were excluded to avoid detection of nonspecific ICBs bound to DCs. The asterisks () represent statistically significant effects (). .

(a)

(b)

(c)